alpha300 R Micro Raman Imaging Spectrometer

The WITec alpha300 R Raman microscope has long been recognized for its cutting-edge imaging technology, and its flexibility, speed, sensitivity, and performance have made it the market leader in confocal Raman imaging.

WITec also maintains a focus on innovation and the introduction of new technologies, which is the basis for the alpha300 R Rapid Raman Imaging technology to remain at the forefront.

The flexible and modular design of the alpha300 R Imaging System enables the combination of different imaging technologies to meet new and expanding needs, providing customers with customized solutions.

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Categories: spectral system, Raman spectroscopy

Description

Main characteristics

Confocal Raman imaging with unprecedented high speed, high sensitivity and high resolution
A complete Raman spectrum is acquired for each sample point in the hyperspectral image.
Excellent horizontal resolution
Excellent longitudinal resolution, ideal for 3D imaging and depth analysis
Ultra-fast Raman imaging with single-spectrum integration time below 1 ms
Spectral acquisition with ultra-high flux lenses for highest sensitivity and best spectral resolution
Non-destructive imaging technology: no need to stain or label samples

Examples of applications

alpha300R stress in diamond

Confocal Raman image of stress changes in a diamond film with color changes in response to stress changes.
Red: compressive strain, blue: tensile strain.

WITec 3D Raman image pollen honey

3D Raman image of pollen in crystallized honey. Scanning points: 150x150x50= 1,125,000 Raman spectra, Scanning area: 50 μmx50 μmx50 μm, Integration time for a single spectrum: 12.2 ms, Total acquisition time: 3 hours and 50 minutes.

alpha300R 3D semiconductor depth scan

Raman longitudinal depth scan of a semiconductor GaN layer. Purple: GaN; green: fluorescence at the edge of the structure; red: material stress.

alpha300R 3D emulsion

3D confocal Raman images of oil (green), alkane (magenta) and water (blue).
Scanning points: 150 × 150 × 23 = 517,500 Raman spectra, scanning area: 30 μm × 30 μm × 11.5 μm, total acquisition time: 23 minutes.

technical specification

Raman routine mode of operation

Raman spectral imaging: one complete Raman spectrum is acquired for each sample point
Manual positioning of samples for scanning in the plane (x-y direction) and longitudinal direction (z direction)
Image Overlay: 3D Confocal Raman Imaging
time series (stats.)
Single-point Raman spectral acquisition
Single-point depth sequence acquisition
Fiber-coupled Ultra-High-Throughput Spectrometer (UHTS), designed for low-light Raman microscopy
confocal fluorescence microscope
open-field microscopy

fundamental characteristic

Research grade 6-port objective rotating wheel optical microscope
Camera System: CCD Camera
LED White Light Source Kohler Lighting
Manual sample positioning in x-y direction
fiber optic coupling

Raman selectable/upgradeable operating modes

Various excitation wavelengths can be selected
A wide range of ultra-high-throughput spectrometers are available (UV, VIS, NIR)
Automated motorized sample positioning and spectra collection with 25 mm panning range (50 mm optional)
Automated Confocal Raman Imaging
Automatic multi-area, multi-point measurement
Fully automated: see details alpha300 apyron
Optional ultra fast Raman imaging
Scalable Drop Shot Fluorescence Applications
Configurable adapters for higher samples
TrueSurface In-depth analysis
autofocus
Options: Dark Field Microscope, Phase Difference Microscope and Differential Interference (DIC) Microscope

Ultra High Throughput Spectrometer UHTS

Optimized spectrometers (UV, VIS or NIR) based on various lenses and excitation wavelengths designed for Raman microscopy and weak signal applications
Fiber-coupled ultra-high throughput optical system
Spectral peaks are symmetrical and free of aberration

software interface

outfit with WITec Professional SoftwareFor instrument control, data acquisition and data processing.